Perutz, M. Haurowitz, F. Gottlieb, A. Hasserodt, U. Rosemeyer, M. Hewitt, J. Guidotti, G. Hill, R. Konigsberg, W. Schroeder, W. Tanford, C. Google Scholar. Bucci, E. Bruice, T. Kekwick, R. Huehns, E. Jones, R. Buettner-Janusch, J. Dayhoff, M. Matsuda, G. Best, J.
Ayre, G. Article Google Scholar. Greer, J. Hayashi, A. Stamatoyannopoulos, G. Clicking on the donut icon will load a page at altmetric. Find more information on the Altmetric Attention Score and how the score is calculated. The oxygen binding properties of vertebrate hemoglobins Hb have been explored in great detail.
In contrast, folding and assembly of these heterotetrameric protein complexes remain poorly understood. Similar to investigations of other multisubunit systems, in vitro Hb refolding experiments are often plagued by aggregation. This technique allows the observation of coexisting subunit combinations, heme binding states, and protein conformers. Hb reassembly is triggered by solvent exchange. Experiments conducted at room temperature provide a low metHb refolding yield. In the absence of aggregation-related interference, Hb assembly follows a symmetric pathway.
Heme-bound monomers then form heterodimers, and ultimately heterodimer association results in native Hb. This work highlights the utility of time-resolved ESI-MS investigations for interrogating the kinetic competition between on-pathway events and aberrant side reactions during the self-assembly of biomolecular complexes.
View Author Information. Fax: E-mail: [email protected]. Mirsky was astonished that I would have the temerity to approach Flexner -- I was a brash young man, I think -- and then astonished that it worked out!
Click images to enlarge. Linus Pauling May 10, Hemoglobin and Sickle Cell Anemia. Shifting Gears and Bridging Disciplines. Early Hemoglobin Investigations. Karl Landsteiner. Right Time and Place. Harvey A. Failures and Successes. Singer and Wells. Oxford and the Alpha-Helix. Theoretical Conclusions. The Genetics of Disease. Subsequent Research. Molecular Diseases. Hope for the Future.
Molecular Medicine. Hemoglobin Structure. Alpha and Beta Chains.
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